These molds were designed by Dr. Satish Kitambi, CEO of K-life solutions whos been working with zebrafish for a long time. One frustrating thing about working with zebrafish is that the process of doing microinjections into the zebrafish embryos is cumbersome and time consuming.
They use agarose gel to line up the embryos for the injection, but to form a hole in the agarose to put the embryos in they use “whatever they have in the lab”, be it lids of plastic tubes or other things lying around. Satish figured they could use neatly designed molds to line up the embryos in a much more thorough way. The idea of the Zebrafish Microinjection mold was born.